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Companion Diagnostic Clinical Trials: Navigating Regulatory Challenges and Opportunities in the US and EU

Written by Nicolas Garret | Sep 29, 2026, 11:08:56 AM

The development of a companion diagnostic (CDx) alongside a medicinal product brings together complex regulatory, clinical, and analytical considerations. While the US and EU share the fundamental concept of a companion diagnostic, the regulatory frameworks and expectations are not identical, and these differences can have a significant impact on clinical trial planning, evidence requirements, timelines, and overall development strategy.

In this blog, we explore key similarities and differences between the US and EU approaches to companion diagnostics, including how a test may be classified, how clinical evidence is considered, and how regulatory interactions can influence development timelines. We also examine practical considerations for planning CDx clinical trials and discuss scenarios that illustrate where differences between the two jurisdictions can create challenges, as well as opportunities to address them early in development.

This content was originally presented as a Solution Circle during the RAPS Convergence 2026.

What makes a test a "companion" diagnostic?

The core idea is the same for both the US and the EU. A companion diagnostic (CDx) provides information that is essential, not merely helpful, for the safe and effective use of a corresponding drug. FDA sets this out in its 2014 guidance, In Vitro Companion Diagnostic Devices, and the EU defines it at Article 2(7) of the IVDR (Regulation (EU) 2017/746).

Unfortunately, the details are not identical.

Where they align - Both definitions cover the two classic use cases: selecting patients likely to benefit and identifying patients at increased risk of serious adverse reactions. This shared core is why most biomarker-driven oncology eligibility tests are treated as CDx in both the US and EU without much friction.

Where they differ - Four gaps matter in practice: 

  1. FDA's monitoring and "adequately studied population" use cases have no clean IVDR equivalent. A dose-monitoring assay that is clearly a CDx for FDA is not a CDx in the EU, but a “normal” class C IVD (classification rule 3 (j)).
  2. FDA explicitly excludes tests that labeling only suggests. A “suggested” test would be considered as a “complementary diagnostic” under the FDA and IVDR has no equivalent scenario. A test that avoided CDx status in the US could still be argued to be "essential" in the EU, particularly if EU labeling is worded more strongly.
  3. IVDR states "before and/or during treatment" explicitly, which may lead notified bodies to read treatment-phase applicability more literally than FDA reviewers.
  4. "Therapeutic product" and "medicinal product" are not perfectly interchangeable terms in theory, which could cause problems between the jurisdictions.

A key takeaway from this discussion is that a device's US classification does not automatically travel to the EU. A single asset can end up on a PMA track in the US and a different conformity route in the EU, with differing levels of evidence, timelines, and engagement strategies. A documented, defensible rationale for each jurisdiction protects you in due diligence and inspection.

US vs. EU

United States - Companion diagnostics typically follow the PMA (Class III) pathway. FDA expects the CDx to be approved, cleared, or granted De Novo contemporaneously with the therapeutic. FDA's 2020 class-labeling guidance also supports a single test labeled across a group of oncology drugs (one diagnostic test, many drugs), which is relevant for NGS panels and multi-drug tests.

European Union - Under IVDR, companion diagnostics default to Class C and require notified body conformity assessment. Under Article 48(3)/(4), the notified body must seek a scientific opinion from EMA (for centrally authorized medicines) or a national competent authority on the suitability of the device for use with the specific medicinal product. This step has no direct FDA comparison.

The structural difference that drives most timeline friction is FDA's culture of parallel, coordinated drug and device review versus the EU's sequential loop for the CDx: notified body, then medicines authority opinion, then CE mark with a completely separate review of the drug. This can make planning key milestone dates difficult when submitting in multiple jurisdictions.

A few principles that we touched on during the Solutions Circle:

  • Use the “final” assay in the registrational trial wherever feasible. When a bridging study is needed instead, it introduces risk from differences in analytical performance, different cutoffs, and potential shifts in patient classification. This would be additional work, but is better than a complete rework or needing to restart the CDx study
  • Establish analytical validation before or alongside clinical use: accuracy, precision/reproducibility, analytical sensitivity and specificity, interference, and stability. Establishing the assay’s performance is crucial in its use in clinical applications or scenarios.
  • Lock (or at least bound) the assay's performance and cutoff early. The drug trial's statistical analysis plan depends on it before biomarker-stratified endpoints can be finalized.
  • Use all communication resources available. Plan a Q-sub with the FDA to discuss your CDx submission, engage the notified in structured dialog early in the process. You may not solve every problem, but good communication can lay solid groundwork.

We worked through two scenarios in the Solution Circle:

Compressed timeline scenario - Breakthrough Therapy designation pulls the filing date forward by six months while the CDx is mid-way through analytical validation and bridging specimens are still being collected. Meeting the new date would mean finalizing the cutoff before the full precision and reproducibility dataset is available.

  • Good practice is to bring the diagnostic partner into regulatory strategy discussions at the same table as clinical and regulatory leads, not after the protocol is locked.
  • Consider timing the CDx sponsor's FDA Pre-Submission alongside the drug sponsor's own milestone meetings so both review teams are aware of each other early.
  • If pursuing simultaneous US and EU filing, map the IVDR notified body consultation timeline against the FDA review clock explicitly, rather than assuming they will run in parallel. Engage early with your notified body and, where relevant, seek EMA or national scientific advice before locking the clinical trial design.

Divergent requirement scenario - A sponsor pursues simultaneous US and EU approval. The PMA is already submitted, but the IVDR consultation process surfaces a request for additional clinical performance data that was not required in the US, putting EU launch timing at risk.

The questions raised by these scenarios are worth considering early in any companion diagnostic development program: At what point could a different regulatory decision have changed the development path? Are the right perspectives represented across clinical, regulatory, biostatistical, and diagnostic teams? And what decisions or regulatory interactions should be prioritized in the next 30 days?

For companies developing companion diagnostics for both the US and EU markets, understanding the differences between the two regulatory frameworks early can help reduce avoidable delays and support a more coordinated development strategy. Building regulatory considerations into clinical trial planning from the outset, establishing clear communication between the drug and diagnostic teams, and engaging with the relevant regulatory bodies early can help identify potential challenges before they become obstacles to development.

Companion diagnostic development continues to evolve, and regulatory expectations can vary depending on the specific drug, diagnostic technology, intended use, and development pathway. The resources below provide further guidance for those looking to explore the regulatory framework and clinical evidence considerations in more detail.

Contact us to discuss the possibilities.

Further reading

  • FDA, In Vitro Companion Diagnostic Devices (August 2014, final)
  • FDA, Principles for Codevelopment of an IVD Companion Diagnostic Device with a Therapeutic Product (July 2016, draft)
  • FDA, Developing and Labeling IVD Companion Diagnostic Devices for a Specific Group of Oncology Therapeutic Products (April 2020, final)
  • FDA, Oncology Drug Products Used with Certain In Vitro Diagnostic Tests: Pilot Program (June 2023)
  • MDCG 2022-2, General Principles of Clinical Evidence for IVDs
  • CTEG/MDCG 2022-10, Q&A on the Interface Between the Clinical Trials Regulation and IVDR
  • EMA, Guidance on the Procedural Aspects for the EMA/NCA Consultation on Companion Diagnostics.